Purification and characterization of .BETA.-D-galactosidase and .ALPHA.-D-mannosidase from papaya (Carica papaya) seeds.
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چکیده
منابع مشابه
Characterization of the lipase from Carica papaya residues Caracterização da lipase de resíduos de Carica papaya
Lipases from vegetable sources have been the focus of intense and growing research. The use of enzymes from plants has the advantage of employing industrial waste products. Brazil is the world’s largest papaya producer and the market for products derived from papaya is growing year after year. In this study, the authors carried out the characterization and partial purification of the lipase obt...
متن کاملCharacterization of a high oleic oil extracted from papaya (Carica papaya L.) seeds Caracterização de um óleo alto oleico extraído de sementes de mamão (Carica papaya L.)
Carica papaya L. seeds have been used for decades in parts of Asia and South America as a vermifugal agent (ADEBIYI; GANESAN ADAIKAN; PRASAD, 2003). In addition, papaya seed preparations have been used in folk medicine due to its abortive properties and to favor a good menstrual flow (RAO; JAMIR, 1982). Apart from these uses, since papaya seeds have a spicy flavor, they are used to adulterate b...
متن کاملExtraction and Characterization of Protease From Senesced Leaves of Papaya (Carica Papaya) and It’s Application
Natural senescence is associated with several fold increase in activity of protease and is characterized by the degradation and loss of proteins therefore plant proteases have been a common studied interestingly. Protease from senesced leaves of the papaya plant (Carica Papaya) was partially purified by ammonium sulfate precipitation. It showed 15 fold purification. Optimum protease activity wa...
متن کاملEXTRACTION AND CHARACTERIZATION OF PECTIN DERIVED FROM PAPAYA (Carica papaya Linn.) PEEL
The aim of study was to extract pectin from papaya peel and study its physico‐chemical characteristics. Pectin was extracted using hydrochloric acid and citric acid, at different time, temperature and pH combinations. Extraction was done at temperatures 60°C, 70°C and 80°C for time 30 mins, 45 mins and 60 mins, and pH 2, 2.5 and 3.0 respectively. Using hydrochloric acid, the yield of pectin was...
متن کاملPurification and properties of alpha-D-mannosidase from rat epididymis.
1. alpha-d-Mannosidase from rat epididymis was purified 300-fold. beta-N-Acetyl-glucosaminidase and beta-galactosidase were removed from the preparation by treatment with pyridine. Zn(2+) was added during the purification to stabilize the alpha-mannosidase. 2. Mammalian alpha-mannosidase is most stable at pH6. At lower pH values it undergoes reversible spontaneous inactivation. The enzyme is al...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1983
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.47.2441